SLIT2 Protein (C-His, Human)

Product Specifications

Abbreviation

SLIT2

Synonyms

SLIL3, Slit-2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human SLIT2 Protein (His Tag) (Cat: 11967-H08H) at 10 μg/mL (100 μL/well) can bind Recombinant Human ROBO1 Protein (ECD, Fc Tag) (Cat: 30073-H02H), the EC50 is 60-180 ng/mL. 2.Loaded Recombinant Human ROBO1 Protein, hFc Tag (Cat. No. 30073-H02H) on proA Biosensor, can bind Recombinant Human SLIT2 Protein, His Tag (Cat. No. 11967-H08H) with an affinity constant of 42.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Leu271-Ala480

Molecular Weight

The recombinant Human SLIT2 consisting of 221 amino acids and has a calculated molecular mass of 24.98 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 23.5 kDa protein in SDS-PAGE under reducing conditions.