SIRPA Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

SIRPA

Synonyms

Bit, CD172a, Idd13.2, Myd1, P84, Ptpns1, SHP-1, SHPS-1, Shps1, SIRP, Sirpa

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized rat CD47-His (Cat:80305-R08H) at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc, The EC50 of mouse SIRPA-Fc is 0.6-1.4 μg/ml. 2.Recombinant Mouse SIRP alpha/SHPS-1 Protein, hFc Tag (Cat. No. 50956-M02H) captured on Protein A chip can bind Recombinant Mouse CD47 Protein, His Tag (Cat. No. 57231-M08H) with an affinity constant of 0.5703 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Asn373

Molecular Weight

The secreted recombinant mouse SIRPA/Fc is a disulfide-linked homodimer. The reduced monomer comprises 583 amino acids and has a calculated molecular mass of 64.9 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA/Fc monomer is approximately 1001-20 kDa in SDS-PAGE under reducing conditions.