SIRPA Protein (C-His, Rat)

Product Specifications

Abbreviation

SIRPA

Synonyms

Bit, Mfr, Ptpns1, SHPS-1, Shps1, Sirp, Sirpa

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat SIRPA-His (Cat:80270-R08H) at 10μg/mL (100μL/well) can bind biotinylated rat CD47-His (Cat:80305-R08H), the EC50 of biotinylated rat CD47-His is 0.1-0.4ug/mL.

Function

Met1-Asn373

Molecular Weight

The recombinant rat SIRPA comprises 353 amino acids and predicts a molecular mass of 39 kDa. The apparent molecular mass of the recombinant protein is approximately 63-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.