SIRPA Protein (C-His, Mouse)

Product Specifications

Abbreviation

SIRPA

Synonyms

Bit, CD172a, Idd13.2, Myd1, P84, Ptpns1, SHP-1, SHPS-1, Shps1, SIRP, Sirpa

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc (Cat:12283-H02H), The EC50 of human CD47-Fc (Cat:12283-H02H) is 0.05-0.13 μg/ml.

Function

Met1-Asn373

Molecular Weight

The secreted recombinant mouse SIRPA comprises 353 amino acids and has a calculated molecular mass of 39.4 kDa. As a result of glycosylation, the apparent molecular mass of mouse SIRPA is approximately 50-70 kDa in SDS-PAGE under reducing conditions.