SIRPA Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

SIRPA

Synonyms

BIT, CD172A, MFR, MYD-1, MYD1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized human CD47-His (Cat: 12283-H08H) at 2 μg/ml (100 μl/well) can bind human SIRPA-Fc (Cat: 11612-H02H1), the EC50 of human SIRPA-Fc is 3-15 ng/mL (QC tested) . 2.Immobilized Recombinant Human SIRP alpha Protein (ECD, Fc Tag) (Cat: 11612-H02H1) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD47 Protein (ECD, His Tag) (Cat: 12283-H08H), the EC50 is 8.8 ng/mL (Routinely tested) .

Function

Met1-Arg370

Molecular Weight

The recombinant human SIRPA consists of 578 amino acids and predicts a molecular mass of 64 kDa. As a result of glycosylation, it migrates as an approximately 77.9 kDa band in SDS-PAGE under reducing conditions.