SIRPA Protein (C-human IgG1-Fc & AVI, Human)

Product Specifications

Abbreviation

SIRPA

Synonyms

BIT, CD172A, MFR, MYD-1, MYD1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2

Host

HEK 293

Tag

C-human IgG1-Fc & AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human CD47 His (Cat:12283-H08H) at 2 μg/mL (100 μL/well) can bind Human SIRP alpha (hFc & AVI Tag), Biotinylated (Cat:11612-H41H-B), the EC50 of Human SIRP alpha (hFc & AVI Tag), Biotinylated (Cat:11612-H41H-B) is 25-150ng/mL.

Function

Met1-Arg370

Molecular Weight

The recombinant human SIRPA consists of 593 amino acids and predicts a molecular mass of 65.85 kDa. It migrates as an approximately 84.19 kDa band in SDS-PAGE under reducing conditions.