SIRPA Protein (C-mouse IgG2a-Fc, Human)

Product Specifications

Abbreviation

SIRPA

Synonyms

BIT, CD172A, MFR, MYD-1, MYD1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2

Host

HEK 293

Tag

C-mouse IgG2a-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized human CD47-His (Cat: 12283-H08H) at 2 μg/ml (100 μl/well) can bind human SIRPa (V8) -mFc (Cat: 30015-H38H), the EC50 of human SIRPa (V8) -mFc is 2-14 ng/mL.

Function

Met1-Arg369 (S44L, S50T, I52T, H54R, V57A)

Molecular Weight

The recombinant human SIRP alphaV8 consists of 572 amino acids and predicts a molecular mass of 63.6 kDa. As a result of glycosylation, it migrates as an approximately 80.4 kDa band in SDS-PAGE under reducing conditions.