SIRPA Protein (C-His, Human)
Product Specifications
Abbreviation
SIRPA
Synonyms
BIT, CD172A, MFR, MYD-1, MYD1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized human CD47 (Cat: 12283-HCCH) at 2 μg/ml (100 μl/well) can bind human SIRPa (V8) -His (Cat: 30015-H08H), the EC50 of human SIRPa (V8) -His is 150-500 ng/mL (QC tested) . 2.Immobilized Recombinant Human SIRP alphaV8 Protein (ECD, His Tag) (Cat: 30015-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD47 Protein (ECD, Fc Tag) (Cat: 12283-H02H), the EC50 is 3.2 ng/mL (Routinely tested) .
Function
Met1-Arg369 (S44L, S50T, I52T, H54R, V57A)
Molecular Weight
The recombinant human SIRP alphaV8 consists of 350 amino acids and predicts a molecular mass of 38.6 kDa. As a result of glycosylation, it migrates as an approximately 49.9 kDa band in SDS-PAGE under reducing conditions.
Species
Human
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