SIRPA Protein (C-His, Human)

Product Specifications

Abbreviation

SIRPA

Synonyms

BIT, CD172A, MFR, MYD-1, MYD1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized human CD47 (Cat: 12283-HCCH) at 2 μg/ml (100 μl/well) can bind human SIRPa (V8) -His (Cat: 30015-H08H), the EC50 of human SIRPa (V8) -His is 150-500 ng/mL (QC tested) . 2.Immobilized Recombinant Human SIRP alphaV8 Protein (ECD, His Tag) (Cat: 30015-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD47 Protein (ECD, Fc Tag) (Cat: 12283-H02H), the EC50 is 3.2 ng/mL (Routinely tested) .

Function

Met1-Arg369 (S44L, S50T, I52T, H54R, V57A)

Molecular Weight

The recombinant human SIRP alphaV8 consists of 350 amino acids and predicts a molecular mass of 38.6 kDa. As a result of glycosylation, it migrates as an approximately 49.9 kDa band in SDS-PAGE under reducing conditions.