SHH Protein (C-His, Mouse)

Product Specifications

Abbreviation

SHH

Synonyms

9530036O11Rik, Dsh, HHG-1, Hhg1, Hx, Hxl3, M100081, Shh, ShhNC

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 1-10 μg/mL.

Function

Met1-Gly198

Molecular Weight

The recombinant mouse SHH comprises 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.