SHH Protein (C-His, Human)

Product Specifications

Abbreviation

SHH

Synonyms

HHG1, HLP3, HPE3, MCOPCB5, ShhNC, SMMCI, TPT

Host

HEK 293

Tag

C-His

Endotoxin

< 50 EU per mg protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 2-10 μg/mL.

Function

Met1-Gly197

Molecular Weight

The recombinant human SHH (aa 1-197) consists of 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 24 kDa in SDS-PAGE under reducing conditions due to glycosylation.