SEZ6 Protein (C-His, Rat)

Product Specifications

Abbreviation

SEZ6

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Rat SEZ6 Protein (ECD, His Tag) (Cat: 85358-R08H) at 2 μg/mL (100 μL/well) can bind Anti-SEZ6 antibody, Human IgG1, the EC50 is 15-45 ng/mL (QC tested) . 2.Loaded anti-SEZ6 antibody, human IgG on proA Biosensor, can bind Recombinant Rat SEZ6 Protein, His Tag (Cat. No. 85358-R08H) with an affinity constant of 83.900 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Leu20-His923

Molecular Weight

The recombinant Rat SEZ6 comprises 915 amino acids and has a predicted molecular mass of 99.47 kDa. It migrates as approximately 192.5 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 155.1 kDa verified by SEC-MALS (Routinely tested) .