SELE Protein (C-His, Rat)
Product Specifications
Abbreviation
SELE
Synonyms
Elam-1, Sele
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to rat E Selectin/Fc Chimera coated plates (2 μg/mL, 100 μL/well), approximately 30%-60% will adhere after 1 hour at 37°C. Optimal dilutions should be determined by each laboratory for each application.
Function
Met1-Pro494
Molecular Weight
The recombinant rat SELE comprises 484 amino acids and predicts a molecular mass of 53 kDa. The apparent molecular mass of the rat SELE is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Species
Rat
Frequently Asked Questions
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