RSPO1 Protein (C-His, Human)

Product Specifications

Abbreviation

RSPO1

Synonyms

CRISTIN3, FLJ40906, RSPO, RSPONDIN

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 98 % as determined by SDS-PAGE

Bioactivity

1. Measured by its ability to bind recombinant mouse CD36 in a functional ELISA. 2.Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 15-150 ng/mL in the presence of 2 ng/mL recombinant Wnt3a.

Function

Met1-Ala146

Molecular Weight

The secreted recombinant human RSPO1 (aa 1-146) comprises 137 amino acids and has a predicted molecular mass of 14 kDa. As a result of glycosylation, rhRSPO1 migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.