ROBO1 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

ROBO1

Synonyms

CPHD8, DUTT1, FLJ21882, NORS, NYS8, SAX3

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human SLIT2 Protein (His Tag) (Cat: 11967-H08H) at 10 μg/mL (100 μL/well) can bind Recombinant Human ROBO1 Protein (ECD, Fc Tag) (Cat: 30073-H02H), the EC50 is 60-180 ng/mL. 2.Loaded Recombinant Human ROBO1 Protein, hFc Tag (Cat. No. 30073-H02H) on proA Biosensor, can bind Recombinant Human SLIT2 Protein, His Tag (Cat. No. 11967-H08H) with an affinity constant of 42.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Pro897

Molecular Weight

The recombinant human ROBO1 consists of 898 amino acids and predicts a molecular mass of 98.89 kDa. It migrates as an approximately 136.91 kDa band in SDS-PAGE under reducing conditions.