REN Protein (C-His, Mouse)

Product Specifications

Abbreviation

REN

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

1. Measured by its ability to cleave the fluorogenic peptide substrate 5FAM/QXLTM 520 (PetiPeterdi, J. et al., 2009, Physiology 24:88.) . The specific activity is > 20 pmoles/min/μg. 2. Immobilized mouse REN1-His (Cat: 50279-M08H) at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His (Cat: 10994-H08H) with a linear range of 31.25-250 ng/ml.

Function

Met1-Arg402

Molecular Weight

The recombinant mouse REN1 consists of 392 amino acids and has a predicted molecular mass of 43.2 kDa. As a result of glycosylation, the apparent molecular mass of rmREN1 is approximately 48-55 kDa in SDS-PAGE under reducing conditions.