RBP4 Protein (C-human IgG1-Fc, Canine)

Product Specifications

Abbreviation

RBP4

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 94 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to bind alltrans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. The 50% binding concentration (BC50) is >5 μM

Function

Met1-Leu201

Molecular Weight

The recombinant canine RBP4 comprises 424 amino acids and has a predicted molecular mass of 48 kDa. The apparent molecular mass of the protein is approximately 53 kDa in SDS-PAGE under reducing conditions due to glycosylation.