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RABV-G Protein (Native, RABV)

Product Specifications

Abbreviation

RABV-G

Host

HEK 293

Tag

Native

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.

Bioactivity

1.Immobilized Recombinant Rabies virus (RABV) Glycoprotein/G Protein (Cat: 40997-VNAH3) at 1 μg/mL (100 μL/well) can bind Anti-RABV Antibody (RVC20), Human IgG1, the EC50 is 4-15 ng/mL. 2.RABV-G- (RVC20) -IgG1 captured on Protein A chip, can bind Rabies virus (RABV) Glycoprotein/G Protein (Cat. No. 40997-VNAH3) with an affinity constant of 0.459 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 3.RABV-G (17C7) -IgG1 captured on Protein A chip, can bind Rabies virus (RABV) Glycoprotein/G Protein (Cat. No. 40997-VNAH3) with an affinity constant of 8.430 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Molecular Weight

It migrates as an approximately 61 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 172.5 kDa verified by SEC-MALS.

Species

RABV

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