PVR Protein (C-His, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized human CD155 (Cat: 10109-H08H) at 2 μg/mL (100 μL/well) can bind human DNAM1 (Cat: 10565-H03H), the EC50 of human DNAM1/CD226 is 50-300 ng/mL. 2.Loaded Human CD226 Protein, hFc Tag (Cat.No.10565-H02H) on ProA Biosensor, can bind human CD155 protein, His Tag (Cat.No.10109-H08H) with an affinity constant of 0.87μM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) . 3.Loaded Recombinant Human TIGIT Protein, hFc Tag (Cat.No. 10917-H02H) on ProA Biosensor, can bind Recombinant Human CD155/PVR Protein, His Tag (Cat.No. 10109-H08H) with an affinity constant of 0.18 uM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human CD155 consists of 334 amino acids and has a predicted molecular mass of 36.5 kDa. As a result of glycosylation, it migrates as approximately 60-65 kDa band in SDS-PAGE under reducing conditions.