PLAT Protein (N-cleavage, Human)

Product Specifications

Abbreviation

PLAT

Synonyms

T-PA, TPA

Host

HEK 293

Tag

N-cleavage

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin. The specific activity is > 300 pmols/min/μg.

Function

Ile311-Pro562

Molecular Weight

The recombinant human tPA β chain consists of 252 amino acids and has a predicted molecular mass of 28 kDa. As a result of glycosylation, the apparent molecular mass of rh tPAβ is approximately 32.6 kDa in SDS-PAGE under non-reducing conditions.