PGA4 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

PGA4

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 80 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2, AnaSpec, Catalog # 27114. The specific activity is > 3000 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)

Function

Met1-Ala388

Molecular Weight

The secreted recombinant human PGA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 614 amino acids and has a predicted molecular mass of 67.3 kDa. The apparent molecular mass of rhPGA/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.