PECAM1 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

PECAM1

Synonyms

CD31, CD31/EndoCAM, endoCAM, GPIIA', PECA1, PECAM-1

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 10E4 cells/well are added to sCD31-Fc coated plates (5 μg/mL and 100 μL/well) in the presence of 10 μg/ml PHA approximately >60% will adhere after 30 minutes at 37°C.

Function

Met1-Lys601

Molecular Weight

The recombinant human CD31/Fc is a disulfide-linked homodimer.The reduced monomer consists of 812 amino acids and predicts a molecular mass of 91.2 kDa. As a result of glycosylation, the rhCD31/Fc monomer migrates as approximately 130-140 kDa band in SDS-PAGE under reducing conditions.