PDGFC Protein (N-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

PDGFC

Synonyms

1110064L01Rik, PDGF-C, Pdgfc, SCDGF, VEGF-E

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 80 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse PDGF-C (Cat:50458-M01H) at 10 μg/mL (100 μl/well) can bind human PDGFRA (Cat:10556-H08H), The EC50 of human PDGFRA (Cat:10556-H08H) is 0.71 μg/mL. 3. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.5-2 μg/mL.

Function

Val235-Gly345

Molecular Weight

The recombinant mouse PDGFC/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 372 amino acids and has a predicted molecular mass of 40.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmPDGFC/Fc monomer is approximately 46 kDa.