PDGFB Protein (N-mouse IgG1-Fc, Cynomolgus)

Product Specifications

Abbreviation

PDGFB

Host

HEK 293

Tag

N-mouse IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PDGFRB-His (Cat:90215-C08H) at 10 μg/ml (100 μl/well) can bind Cynomolgus S4-mFc3-PDGFB, The EC50 of Cynomolgus S4-mFc3-PDGFB is 3.6-7.4 ng/ml.

Function

Ser82-Thr190

Molecular Weight

The recombinant cynomolgus PDGFB is a disulfide-linked homodimer. The reduced monomer comprises 345 amino acids and has a calculated molecular mass of 38.9 KDa. The apparent molecular mass of it is approximately 43 and 32 KDa in SDS-PAGE.