PDGFA Protein (N-His, Human)

Product Specifications

Abbreviation

PDGFA

Synonyms

PDGF-A, PDGF1

Host

Yeast

Tag

N-His

Endotoxin

< 50 EU per mg protein

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Western blot analysis of extracts from serum-starved NIH-3T3, untreated (-) ; treated with PDGFA (Cat# 13081-H07Y) (5 μg/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody (Cat# 110591-R0113) at 1:2000 dilution (upper) or anti-Akt antibody (lower) (Routinely tested) . 2.Immobilized Recombinant Human PDGFRB Protein (Fc Tag) (Cat:10514-H02H1) at 2 μg/mL (100 μL/well) can bind Recombinant Human PDGFA / PDGF-1 protein (His Tag) (Cat:13081-H07Y), the EC50 is 5-15 ng/mL (QC tested) . 3.Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 20-100ng/ml (Routinely tested) .

Function

Ser87-Thr211

Molecular Weight

The recombinant human PDGFA consists of 140 amino acids and predicts a molecular mass of 16.3 kDa. The molecular weight of this protein is around 41.4 kDa verified by SEC-MALS (Routinely tested) .

Species

Human