PDCD1 Protein (C-mouse IgG2a-Fc, Human)

1.Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (mFc Tag) (Cat: 10377-H38H-UE) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10084-H41H-B), the EC50 is 12-40 ng/mL (Routinely tested) . 2.Loaded Recombinant Human PD1 / PDCD1 / CD279 Protein (mFc Tag) (Cat. No. 10377-H38H-UE) on ProA Biosensor, can bind Recombinant Human B7-DC / PD-L2 / CD273 Protein (His Tag) (Cat. No. 10292-H08H) with an affinity constant of 5.34 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Anti-PD1 (Research Grade Pembrolizumab Biosimilar) captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Human PD1 / PDCD1 / CD279 Protein (mFc Tag) (Cat: 10377-H38H-UE) with an affinity constant of 0.701 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human PDCD1 consists of 381 amino acids and predicts a molecular mass of 42.7 kDa. As a result of glycosylation, human PDCD1 migrates as an approximately 55-66 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 109.0 kDa verified by SEC-MALS (Routinely tested) .