PDCD1 Protein (C-mouse IgG1-Fc, Human)

1.Immobilized PD-1 Protein, Human, Recombinant (ECD, mFc Tag) (Cat:10377-H05H) at 2 μg/mL (100 μL/well) can bind PD-L1 Protein, Human, Recombinant (ECD, His Tag), Biotinylated (Cat:10084-H08H-B), the EC50 is 150-800 ng/mL. 2. Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (ECD, Fc Tag) (Cat: 10377-H05H) at 2 μg/mL (100 μL/well) can bind Anti-PD1 Antibody, Human IgG4 (Nivolumab), the EC50 is 20-60 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (ECD, Fc Tag) (Cat: 10377-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant Human B7-DC / PD-L2 / CD273 / PDCD1LG2 Protein (Fc Tag) (Cat: 10292-H02H), the EC50 is 15-45 ng/mL (Routinely tested) . 4.Loaded Recombinant Human PD-1 Protein, mFc Tag (Cat. No. 10377-H05H) on AMC Biosensor, can bind Recombinant Human PD-L2/B7-DC Protein, His Tag (Cat. No. 10292-H08H) with an affinity constant of 58.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5.Loaded Recombinant Human PD-1 Protein (ECD, mFc Tag) (Cat. No. 10377-H05H) on AMC Biosensor, can bind Recombinant Human PD-L1/B7-H1 Protein (ECD, His Tag) (Cat. No. 10084-H08H) with an affinity constant of 5.820 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human PDCD1 consists of 377 amino acids and predicts a molecular mass of 42.3 kDa. As a result of glycosylation, it migrates as an approximately 58.9 kDa band in SDS-PAGE under reducing conditions.