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PDCD1 Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

PDCD1

Synonyms

Ly101, PD-1, Pd1, Pdc1, Pdcd1

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Mouse PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat:50124-M02H) at 2μg/mL (100 μL/well) can bind Recombinant Mouse PD-L1/B7-H1/CD274 Protein (Fc&AVI Tag), Biotinylated (Cat:50010-M41H-B), with a linear range of 0.16-2.5 μg/mL (QC tested) . 2.Recombinant Mouse PD-1 Protein , hFc Tag (Cat. No. 50124-M02H) captured on Protein A chip can bind Recombinant Mouse PD-L1/B7-H1, His Tag (Cat. No. 50010-M08H) with an affinity constant of 2.489 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Loaded Recombinant Mouse PD-1 Protein, hFc Tag (Cat. No. 50124-M02H) on proA Biosensor, can bind Recombinant Mouse PD-L2/B7-DC Protein, His Tag (Cat. No. 50804-M08H) with an affinity constant of 80.700 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Mouse PD-L1/B7-H1 Protein, His Tag (Cat. No. 50010-M08H) captured on CM5 chip via Anti-his antibody, can bind Recombinant Mouse PD-1 Protein, hFc Tag (Cat. No. 50124-M02H) with an affinity constant of 1.117 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Gln167

Molecular Weight

The recombinant mouse PDCD1 consists of 381 amino acids and predicts a molecular mass of 42.9 kDa. The molecular weight of this protein is around 111.2 kDa verified by SEC-MALS (Routinely tested) .

Species

Mouse

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