PDCD1 Protein (C-human IgG1-Fc & AVI, Human)

1.Immobilized Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat: 10377-H41H-B) at 2 μg/mL (100 μl/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 40-130 ng/mL (QC tested) . 2.Immobilized Anti-PD1 (Research Grade Nivolu*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 0.6-2 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human B7-DC / PD-L2 / CD273 / PDCD1LG2 Protein (Fc Tag) (Cat: 10292-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 3-10 ng/mL (Routinely tested) . 4.Immobilized Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Mouse B7-DC / PD-L2 / CD273 Protein (His Tag) (Cat: 50804-M08H), the EC50 is 13.3 ng/mL (Routinely tested) . 5.Serial dilutions of Anti-PD1 (Research Grade Nivolu*mab Biosimilar) were added into Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B) : Recombinant Human PD-L1 / B7-H1 / CD274 Protein (ECD, mFc Tag) (Cat: 10084-H05H) binding reactions. The half maximal inhibitory concentration (IC50) is 0.3 μg/mL (Routinely tested) .

The recombinant human PDCD1 consists of 396 amino acids and predicts a molecular mass of 44.49 kDa. As a result of glycosylation, it migrates as an approximately 61.93 KDa band in SDS-PAGE under reducing conditions.