PDCD1 Protein (C-His, Human)

Product Specifications

Abbreviation

PDCD1

Synonyms

CD279, hPD-1, hPD-l, hSLE1, PD-1, PD1, SLEB2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human PD1 / PDCD1 Protein (His Tag), Biotinylated (Cat: 10377-H08H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat: 10084-H02H), the EC50 is 30-100 ng/mL (QC tested) . 2.Loaded Anti-human PD1 antibody, IgG4 on ProA Biosensor, can bind Biotinylated Recombinant Human PD1 Protein, His Tag, Biotinylated (Cat. NO. 10377-H08H-B) with an affinity constant of 4.22 nM as determined in a BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Captured Anti-human PD1 antibody, IgG4 on Protein A Chip can bind Biotinylated Human PD1 Protein, His Tag, Biotinylated (Cat. NO. 10377-H08H-B) with an affinity constant of 13.16 nM as determined in a SPR assay (Biacore T200) (Routinely tested) .