PCSK9 Protein (C-His, Rhesus)

Product Specifications

Abbreviation

PCSK9

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Immobilized Evolocumab derived anti-human PCSK9 antibody, human IgG2 (Cat: 68078-H001) at 2 μg/mL (100 μL/well) can bind Recombinant Rhesus PCSK9 / NARC1 Protein (His Tag) (Cat: 11054-K08H), the EC50 is 2.0-6.1 ng/mL (QC tested) .

Function

Met1-Gln692

Molecular Weight

The recombinant Rhesus PCSK9 consists of 673 amino acids and predicts a molecular mass of 72.7 kDa. As a result of glycosylation and proteolytic digestion, it migrates as doublet with apparent molecular mass of 20 kDa and 62 kDa corresponding to the pro domain and mature form respectively in SDS-PAGE under reducing conditions.