PCSK9 Protein (C-AVI & His, Human)

Product Specifications

Abbreviation

PCSK9

Synonyms

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PSEC0052

Host

HEK 293

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Measured by its ability to bind recombinant LDLR-His (Cat:10231-H08H) in a functional ELISA. 2.Loaded Biotinylated Recombinant Human PCSK9 Protein, His & AVI Tag (Cat. No. 29698-H27H-B) on SA Biosensor, can bind Recombinant Human LDLR/LDL Receptor Protein, mFc Tag (Cat. No. 10231-H05H) with an affinity constant of 12.900 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Gln692

Molecular Weight

The recombinant human PCSK9 consists of 688 amino acids and predicts a molecular mass of 74.3 kDa. As a result of glycosylation and proteolytic digestion, it migrates as doublet with apparent molecular mass of 18 kDa and 60 kDa corresponding to the pro domain and mature form respectively in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 77.9 kDa verified by SEC-MALS (Routinely tested) .