PCSK9 Protein (C-His, Human)

Product Specifications

Abbreviation

PCSK9

Synonyms

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PSEC0052

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human PCSK9 Protein (His Tag) (Cat: 29698-H08H2) at 5 μg/mL (100 μL/well) can bind Recombinant Human LDL Receptor Protein (Fc Tag) (Cat: 10231-H05H), the EC50 is 250-750 ng/mL. 2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag (Cat. No. 10231-H05H) on proA Biosensor, can bind Recombinant Human PCSK9 Protein, His Tag (Cat. No. 29698-H08H2) with an affinity constant of 12.800 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human LDLR/LDL Receptor Protein (Fc Tag) (Cat: 10231-H02H) at 2 μg/mL (100μL/well) can bind Recombinant Human PCSK9 / NARC1 (D374Y) Protein (His Tag) (Cat: 29698-H08H1), the EC50 is 8-25 ng/mL. Recombinant Human PCSK9 Protein (wild type, His Tag) (Cat: 29698-H08H2) is verified bind to Human LDLR/LDL Receptor Protein (Fc Tag) (Cat: 10231-H02H) wtih lower affinity (Routinely tested) . 4.Immobilized Recombinant Human PCSK9 Protein (His Tag) (Cat: 29698-H08H2) at 1 μg/mL (100 μl/well) can bind Anti-PCSK9 (Research Grade Evolocu*mab Biosimilar), the EC50 is 2.2 ng/mL (Routinely tested) .

Function

Gln31-Gln692

Molecular Weight

The recombinant Human PCSK9 comprises 681 amino acids and has a predicted molecular mass of 73.03 kDa. It migrates as approximately 60.7 kDa and 18.9 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 76.2 kDa verified by SEC-MALS (Routinely tested) .