NTRK1 Protein (N-human IgG1-Fc, Canine)

Product Specifications

Abbreviation

NTRK1

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit NGF-induced proliferation of TF‐1 human erythroleukemic cells. The ED50 for this effect is typically 2-8 μg/mL in the presence of 10 ng/mL of recombinant human NGF.

Function

Pro285-Lys410

Molecular Weight

The recombinant canine NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 386 amino acids and has a predicted molecular mass of 42.3 kDa. The apparent molecular mass of the protein is approximately 55-61 kDa in SDS-PAGE under reducing conditions.