NTRK1 Protein (C-human IgG1-Fc & His, Human)

Product Specifications

Abbreviation

NTRK1

Synonyms

MTC, p140-TrkA, TRK, Trk-A, TRK1, TRKA

Host

HEK 293

Tag

C-human IgG1-Fc & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.04-0.3 μg/ml in the presence of 10 ng/ml of recombinant human NGF.

Function

Met1-Pro382

Molecular Weight

The recombinant human NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 598 amino acids and has a predicted molecular mass of 66 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NTRK1/Fc monomer is approximately 85-95 kDa due to glycosylation.