NT5E Protein (C-His, Rat)

Product Specifications

Abbreviation

NT5E

Synonyms

CD73, Nt5, Nt5e, Nte

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP) . The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996) . The specific activity is >15,000 pmol/min/μg.

Function

Met1-Lys549

Molecular Weight

The recombinant rat NT5E comprises 532 amino acids and predicts a molecular mass of 59.4 kDa. The apparent molecular mass of the recombinant protein is approximately 59 kDa in SDS-PAGE under reducing conditions due to glycosylation.