NT5E Protein (C-His, Human)

1.Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) (Cat. 10904-H08H) bound MEDI9447 was 0.3 nM (Routinely tested) . 2.Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP) . The specific activity is > 15,000 pmoles/min/μg (QC tested) . 3.Immobilized Recombinant Human CD73 / NT5E Protein (His Tag) (Cat: 10904-H08H) at 1 μg/mL (100 μL/well) can bind Anti-NT5E (Research Grade Oleclumab Biosimilar), the EC50 is 0.8-3.0 ng/mL (QC tested) .

The secreted recombinant human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa. It migrates as an approximately 63 kDa protein due to glycosylation by SDS-PAGE under reducing conditions. The molecular weight of this protein is around 125.4 kDa kDa verified by SEC-MALS (Routinely tested) .