NRG1 Protein (N-human IgG1-Fc, Human)

1. Measured by its binding ability in a functional ELISA. 2. Immobilized Rhesus ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.58 μg/mL. 3. Immobilized human ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 μg/mL. 4.Human breast cancer organoids were cultured with FGF7 (Cat#10210-H07E), RSPO1 (Cat#11083-HNAS), IGF1 (Cat#10598-HNAE), EGF (Cat#50482-MNCH), NRG1 Beta 1 (Cat#11609-H01H), NOG (Cat#50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology. 5.Human breast organoids were cultured with IGF1 (Cat#10598-HNAE), NRG1 Beta 1 (Cat#11609-H01H) . (Routinely tested) . Data provided by D1 Medical Technology. 6.Human ovarian organoids were cultured with IGF1 (Cat#10598-HNAE), NRG1 Beta 1 (Cat#11609-H01H), RSPO1 (Cat#11083-HNAS), EGF (Cat#50482-MNCH), NOG (50688-M02H) . (Routinely tested) . Data provided by D1 Medical Technology.

The recombinant human NRG1 (aa176-246) /Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 331 amino acids and has a calculated molecular mass of 36.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NRG1/Fc monomer is approximately 38 kDa.