NOV Protein (C-His, Human)

Product Specifications

Abbreviation

NOV

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 94 % as determined by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cells. When cells are added to CCN3-coated plates (10 μg/ml, 100 μl/well), >50% cells will adhere specifically after 60 minutes at 37 °C .

Function

Met1-Met357

Molecular Weight

The secreted recombinant human NOV consists of 337 amino acids and predicts a molecular mass of 37 kDa. It migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.