NOTCH1 Protein (C-His, Mouse)

Product Specifications

Abbreviation

NOTCH1

Synonyms

9930111A19Rik, lin-12, Mis6, Motch, N1, Notch1, Tan1

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 82 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 (cat: 10171-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Immobilized mouse DLL4-his (Cat:50640-M08H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL.

Function

Met1-Gln526

Molecular Weight

The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.