NOTCH1 Protein (C-human IgG1-Fc, Human)

1.Immobilized Recombinant Human DLL4 / Delta4 Protein (His Tag) (Cat: 10171-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human NOTCH1 Protein (Fc Tag) (Cat: 10954-H02H), the EC50 is 20-60 ng/mL. 2.Loaded Recombinant Human NOTCH1 Protein, hFc Tag (Cat. No. 10954-H02H) on ProA Biosensor, can bind Recombinant Human DLL1 Protein, His Tag (Cat. No. 11635-H08H) with an affinity constant of 0.889 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human NOTCH1 Protein, hFc Tag (Cat. No. 10954-H02H) on ProA Biosensor, can bind Recombinant Human DLL4 Protein, His Tag (Cat. No. 10171-H08H) with an affinity constant of 51.7nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Human NOTCH1 Protein, hFc Tag (Cat. No. 10954-H02H) captured on Protein A chip can bind Recombinant Human DLL4 Protein, His Tag (Cat. No. 10171-H08H) with an affinity constant of 1.356 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 5.Recombinant Human NOTCH1 Protein, hFc Tag (Cat. No. 10954-H02H) captured on Protein A chip can bind Biotinylated Recombinant Human DLL4 Protein, His & AVI Tag (Cat. No. 10171-H49H-B) with an affinity constant of 0.147 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human NOTCH1 consists of 741 amino acids and predicts a molecular mass of 79.7 kDa. It migrates as approximately 108.3 kDa band in SDS-PAGE under reducing conditions.