NOG Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

NOG

Synonyms

SYM1, SYNS1, SYNS1A

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.1-0.6 μg/mL in the presence of 25 ng/mL of hBMP4.

Function

Met1-Cys232

Molecular Weight

The recombinant human Noggin/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. Each monomer comprises 443 amino acids and has a predicted molecular mass of 49.8 kDa. As a result of glycosylation, the apparent molecular mass of rhNoggin/Fc monomer is approximately 58-62 kDa in SDS-PAGE under reducing conditions.