NOG Protein (C-His, Human)

Product Specifications

Abbreviation

NOG

Synonyms

SYM1, SYNS1, SYNS1A

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 10-60 ng/mL in the presence of 25 ng/mL of recombinant human BMP4.

Function

Met1-Cys232

Molecular Weight

The recombinant human Noggin comprises 216 amino acids after removal of the signal peptide and has a predicted molecular mass of 24.6 kDa. As a result of glycosylation, rhNoggin migrates as an approximately 30 kDa bnd in SDS-PAGE under reducing conditions.