NGFR Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

NGFR

Synonyms

LNGFR, Ngfr, p75, p75NGFR, p75NTR, Tnfrsf16

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 2 ng/mL Recombinant mouse NGF.

Function

Met1-Asn253

Molecular Weight

The secreted recombinant mouse NGFR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a calculated molecular mass of 50.6 kDa. The apparent molecular mass of rmNGFR/Fc monomer is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.