NECTIN1 Protein (C-human IgG1-Fc, Human)

1.Immobilized NECTIN4/Nectin 4 Protein, Human, Recombinant (ECD, His Tag) (Cat:19771-H08H) at 2 μg/ml (100 μl/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag) (Cat:11611-H02H), the EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag) is 20-120 ng/mL. 2.Loaded Recombinant Human Nectin-1/PVRL1 Protein, hFc Tag (Cat. No. 11611-H02H) on ProA Biosensor, can bind Recombinant Human Nectin-4 Protein, His Tag (Cat. No. 19771-H08H) with an affinity constant of 0.32 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human PVRL1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 545 amino acids and predictes a molecular mass of 61 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPVRL1/Fc monomer is approximately 75-85 kDa due to glycosylation.