NECTIN1 Protein (C-His, Human)

Product Specifications

Abbreviation

NECTIN1

Synonyms

CD111, CLPED1, ED4, HIgR, HV1S, HVEC, nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK-12

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 98 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. Immobilized PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.4-800 ng/ml. 2. Immobilized Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10852-H02H) at 2 μg/ml (100 μl/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag) (Cat:11611-H08H), the EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag) is 150-700 ng/mL.

Function

Met1-Thr334

Molecular Weight

The recombinant human PVRL1 consists of 315 amino acids and predictes a molecular mass of 35.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPVRL1 is approximately 45-50 kDa due to glycosylation.