NBL1 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

NBL1

Synonyms

D1S1733E, DAN, DAND1, NB, NO3

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.2-1.2 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.

Function

Met1-Asp180

Molecular Weight

The recombinant human DAN/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and has a calculated molecular mass of 44.4 kDa. As a result of glycosylation, rh DAN/Fc monomer migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.