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MPL Protein (C-His, Human)

Product Specifications

Abbreviation

MPL

Synonyms

C-MPL, CD110, MPLV, THCYT2, THPOR, TPOR

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1. Recombinant Human TPO/Thrombopoietin Protein, hFc Tag (Cat. No. 10381-H02H) captured on Protein A chip can bind Recombinant Human TPO-R Protein, His Tag (Cat. No. 10443-H08H) with an affinity constant of 12.3 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 2. Loaded Recombinant Human TPO/Thrombopoietin Protein, hFc Tag (Cat. No. 10381-H02H) on ProA Biosensor, can bind Recombinant Human TPO-R Protein, His Tag (Cat. No. 10443-H08H) with an affinity constant of 9.06 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Trp491

Molecular Weight

The recombinant Human MPL consists of 477 amino acids and predicts a molecular mass of 54 kDa. It migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.

Species

Human

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