MME Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

MME

Synonyms

CALLA, CD10, CMT2T, EPN, NEP, SCA43, SFE

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp) -OH, (R&D Systems, Catalog # ES005) . The specific activity is >1000 pmoles/min/μg.

Function

Tyr52-Trp750

Molecular Weight

The recombinant human MME/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 936 amino acids and predicts a molecular mass of 106 kDa. As a result of glycosylation, the rh Fc monomer migrates as approximately 120-130 kDa band in SDS-PAGE under reducing conditions.