MET Protein (C-His, Canine)

Product Specifications

Abbreviation

MET

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized human HGF (Cat:10463-HNAS) at 10 μg/mL (100 μl/well) can bind canine c-MET (Cat:70008-D08H), The EC50 of canine c-MET (Cat:70008-D08H) is 7 ng/mL.

Function

Met1-Leu935

Molecular Weight

The recombinant canine c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 922 amino acids and has a predicted molecular mass of 103 (α =33 + β =70) kDa. As a result of glycosylation, the apparent molecular mass of the canine c-Met is approximately 42-47 kDa and 85-95 kDa respectively in SDS-PAGE under reducing conditions.